Details

Autor(en) / Beteiligte

• Suzuki, Hideaki
• Koizumi, Hiroki
• Ikezaki, Shoji
• Tabata, Takahisa
• Ohkubo, Jun-ichi
• Kitamura, Takuro
• Hohchi, Nobusuke

Titel

Electrical Impedance and Expression of Tight Junction Components of the Nasal Turbinate and Polyp

Ist Teil von

• O.R.L. Journal for oto-rhino-laryngology and its related specialties, 2016-01, Vol.78 (1), p.16-25

Ort / Verlag

Basel, Switzerland: S. Karger AG

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Purpose: We investigated the electrical impedance and expression of tight junction components of the turbinate mucosa, nasal polyp, and normal skin. Procedures: The inferior turbinate and nasal polyp of patients with chronic rhinosinusitis and the postauricular skin of patients with otitis media were examined. Electrical impedance was measured in vivo using a tissue conductance meter. Expressions of claudin-1 and tricellulin were examined by fluorescence immunohistochemistry and quantitative RT-PCR. Results: Electrical impedance was higher in the skin than in the turbinate and polyp, but did not differ between the turbinate and polyp. Immunoreactivities for claudin-1 and tricellulin were seen in the epithelial/epidermal layer. Expression of claudin-1 was higher in the skin than in the turbinate and polyp. The polyp tended to show higher expression of claudin-1 but showed lower expression of tricellulin than the turbinate. The ratio of claudin-1 to tricellulin was highest in the skin and lowest in the turbinate. The correlation between expressions of the two tight junction components was strongly positive in the skin (r = 0.964) and negative (r = -0.527) in the turbinate and polyp. Conclusions: These results suggest that the roles of claudin-1 and tricellulin in barrier function may be complementary, and may thereby maintain a constant level of permeability of the mucosal tissues.