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Emergency workers are exposed to chronic and acute emotionally demanding stressors. Fortunately, they have some important psychological resources that can buffer the negative effects deriving from the exposure to occupational stressors. In particular, job, organizational, and family support can protect rescuers against negative health effects, such as burnout and vicarious traumatization (VT). The aim of this study was to investigate the role of perceived social support as a protective factor against negative outcomes in a sample of Italian rescuers. Of the present sample (N = 782), 70.3% were ambulance operators, mainly volunteers (65.9%). They filled in a paper-and-pencil questionnaire aimed at investigating VT, burnout, job support from colleagues and superiors, organizational support in terms of affective commitment, and family support. Stepwise multiple linear regression analyses verified the influence exerted by affective commitment and social support on well-being dimensions. Results showed that affective commitment has the most significant influence on all burnout and VT dimensions, whereas family support has the weakest influence. Our findings confirm that affective commitment is an important coping resource that protects individuals against negative effects and that social support at work is associated with some dimensions of burnout and posttraumatic symptoms. The main result of the present study concerns the important role of affective commitment as a protective resource against negative psychological effects. Therefore, work environments organized in a way that employees can feel affectively committed to their organization may function as a buffer against negative outcomes, especially if it is associated with a strong social support from colleagues.