Details

Autor(en) / Beteiligte

• Dankers, W.
• van Hamburg, J.P.
• Asmawidjaja, P.S.
• Davelaar, N.
• Wen, K.
• Mus, A.M.C.
• Colin, E.M.
• van Leeuwen, J.P.T.M.
• Hazes, J.M.W.
• Lubberts, E.

Titel

OP0141 1,25(OH)2D3 modulates gene expression involved in cytokine production, proliferation, survival and migration of TH17 cells from patients with rheumatoid arthritis

Ist Teil von

• Annals of the rheumatic diseases, 2013-06, Vol.71 (Suppl 3), p.101-101

Ort / Verlag

London: BMJ Publishing Group Ltd and European League Against Rheumatism

Erscheinungsjahr

2013

Quelle

BMJ Journals Archiv - DFG Nationallizenzen

Beschreibungen/Notizen

• Background Vitamin D has suppressive effects on autoimmune diseases, such as rheumatoid arthritis (RA). Within these diseases, T-helper-17 (Th17) cells have been implicated toplay a crucial role in the development and progression of chronic inflammation. Recently, we have found that the active vitamin D compound, 1,25(OH)2D3, has direct suppressive effects on both human and mouse Th17 cytokine expression and activity. Objectives Using gene-expression profiling, we aim to identify molecular targets of 1,25(OH)2D3 signaling underlying the suppressive action of 1,25(OH)2D3 in Th17 cells from patients with RA. Methods Primary Th17 cells were sorted from peripheral blood of treatment naïve patients with early RA and cultured with or without 1,25(OH)2D3. From these cultures gene-expression profiles were generated. Expression of genes of interest was confirmed by Q-PCR and/or specific ELISA. Results In the presence of 1,25(OH)2D3, protein expression of Th17 associated cytokines IL-17A and IL-22 was inhibited, while in contrast the anti-inflammatory cytokine IL-10 was induced. These findings were supported by the gene-expression profiles from these cultures. Furthermore, 1,25(OH)2D3 inhibited transcription of the cytokine receptors IL-23R and IL-7R, which are involved in Th17 survival and proliferation. Chemokines CCL20 and CXCL10 were down-regulated and chemokine receptors CCR2, CXCR6, CXCR3 and CCR10 were up-regulated. Importantly, Rorgt, which is critically involved in Th17 differentiation and function and the cell-size regulator and oncogene, c-Myc were down-regulated by 1,25(OH)2D3. Conclusions From these findings, we concluded that 1,25(OH)2D3 modulates the expression of genes involved in cytokine production, proliferation, survival and migration of Th17 cells. These data indicate that 1,25(OH)2D3 not only suppresses Th17 cell activity but also regulates migration of these cells to sites of tissue inflammation in RA. Disclosure of Interest None Declared