Details

Autor(en) / Beteiligte

• Evans, J C
• Mccarthy, J
• Torres-fuentes, C
• Cryan, J F
• Ogier, J
• Darcy, R
• Watson, R W
• O'driscoll, C M

Titel

Cyclodextrin mediated delivery of NF-[kappa]B and SRF siRNA reduces the invasion potential of prostate cancer cells in vitro

Ist Teil von

• Gene therapy, 2015-10, Vol.22 (10), p.802

Ort / Verlag

Houndmills: Nature Publishing Group

Erscheinungsjahr

2015

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Prostate cancer is the most common cancer in men of the western world. To date, no effective treatment exists for metastatic prostate cancer and consequently, there is an urgent need to develop new and improved therapeutics. In recent years, the therapeutic potential of RNA interference (RNAi) has been extensively explored in a wide range of diseases including prostate cancer using numerous gene delivery vectors. The aims of this study were to investigate the ability of a non-viral modied cyclodextrin (CD) vector to deliver siRNA to the highly metastatic PC-3 prostate cancer cell line, to quantify the resulting knockdown of the two target genes (RelA and SRF) and to study the effects of the silencing on metastasis. Data from a Matrigel in vitro invasion assay indicated that the silencing of the target genes achieved by the CD vector resulted in signicant reductions (P = 0.0001) in the metastatic potential of these cells. As the silencing of these target genes was shown not to have a negative impact on cell viability, we hypothesise that the mechanism of invasion inhibition is due, in part, to the signicant reduction observed (P 0.0001) in the level of pro-inammatory cytokine, MMP9, which is known to be implicated in the metastasis of prostate cancer.