Details

Autor(en) / Beteiligte

• Tam, Jacqueline Chor Wing
• Ko, Chun Hay
• Lau, Kit Man
• To, Ming Ho
• Kwok, Hin Fai
• Siu, Wing Sum
• Lau, Ching Po
• Chan, Wai Yee
• Leung, Ping Chung
• Fung, Kwok Pui
• Lau, Clara Bik San

Titel

Enumeration and functional investigation of endothelial progenitor cells in neovascularization of diabetic foot ulcer rats with a Chinese 2-herb formula æºå½¢èzè½å

Ist Teil von

• Journal of diabetes, 2015-09, Vol.7 (5), p.718

Ort / Verlag

Hoboken: John Wiley & Sons, Inc

Erscheinungsjahr

2015

Quelle

Wiley Online Library Journals

Beschreibungen/Notizen

• Backbround We investigated the effect of a Chinese 2-herb formula (NF3) on the enumeration and angiogenic differentiation of endothelial progenitor cells (EPCs) in diabetic foot ulcer rats. Methods EPCs and stromal cell-derived factor-1[alpha] (SDF-1[alpha]) were quantified by flow cytometry and ELISA, respectively. In vitro angiogenesis assays included proliferation, adhesion, migration and tube formation. Results Our result demonstrated that NF3 (0.98g/kg) could significantly enhance the circulating CD34+/VEGFR2+/CD45- EPCs levels in diabetic foot ulcer rats by 60% (P<0.05) through the partial elevation of SDF-1[alpha], restoring the mobilization ability of EPCs for wound neovascularization. We successfully isolated the BM-derived EPCs to study their angiogenic potential after NF3 treatment. BM-derived EPCs significantly expressed cell surface markers of CD34, CD146 and VEGFR2 (P<0.05-0.01). NF3 could significantly stimulate the proliferation and attachment ability of EPCs dose-dependently (P<0.01-0.001). Besides, NF3 could significantly augment EPCs migration (P<0.001) and tube formation (P<0.01-0.001). Conclusions NF3 modulated diabetic wound healing through regulation of systemic EPCs level and increase in local vascular formation.