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Autor(en) / Beteiligte
Titel
Enumeration and functional investigation of endothelial progenitor cells in neovascularization of diabetic foot ulcer rats with a Chinese 2-herb formula æºå½¢èzè½å
Ist Teil von
  • Journal of diabetes, 2015-09, Vol.7 (5), p.718
Ort / Verlag
Hoboken: John Wiley & Sons, Inc
Erscheinungsjahr
2015
Quelle
Wiley Online Library Journals
Beschreibungen/Notizen
  • Backbround We investigated the effect of a Chinese 2-herb formula (NF3) on the enumeration and angiogenic differentiation of endothelial progenitor cells (EPCs) in diabetic foot ulcer rats. Methods EPCs and stromal cell-derived factor-1[alpha] (SDF-1[alpha]) were quantified by flow cytometry and ELISA, respectively. In vitro angiogenesis assays included proliferation, adhesion, migration and tube formation. Results Our result demonstrated that NF3 (0.98g/kg) could significantly enhance the circulating CD34+/VEGFR2+/CD45- EPCs levels in diabetic foot ulcer rats by 60% (P<0.05) through the partial elevation of SDF-1[alpha], restoring the mobilization ability of EPCs for wound neovascularization. We successfully isolated the BM-derived EPCs to study their angiogenic potential after NF3 treatment. BM-derived EPCs significantly expressed cell surface markers of CD34, CD146 and VEGFR2 (P<0.05-0.01). NF3 could significantly stimulate the proliferation and attachment ability of EPCs dose-dependently (P<0.01-0.001). Besides, NF3 could significantly augment EPCs migration (P<0.001) and tube formation (P<0.01-0.001). Conclusions NF3 modulated diabetic wound healing through regulation of systemic EPCs level and increase in local vascular formation.
Sprache
Englisch
Identifikatoren
ISSN: 1753-0393
eISSN: 1753-0407
DOI: 10.1111/1753-0407.12230
Titel-ID: cdi_proquest_journals_1701643702
Format
Schlagworte
Diabetes, Feet, Foot diseases, Ulcers

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