Details

Autor(en) / Beteiligte

• Jung, Hye-won
• Park, Inae
• Ghil, Sungho

Titel

Cannabinoid receptor activation inhibits cell cycle progression by modulating 14-3-3[beta]

Ist Teil von

• Cellular & molecular biology letters, 2014-09, Vol.19 (3), p.347

Ort / Verlag

Wrocław: BioMed Central

Erscheinungsjahr

2014

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Cannabinoids display various pharmacological activities, including tumor regression, anti-inflammatory and neuroprotective effects. To investigate the molecular mechanisms underlying the pharmacological effects of cannabinoids, we used a yeast two-hybrid system to screen a mouse brain cDNA library for proteins interacting with type 1 cannabinoid receptor (CB1R). Using the intracellular loop 3 of CB1R as bait, we identified 14-3-3[beta] as an interacting partner of CB1R and confirmed their interaction using affinity-binding assays. 14-3-3[beta] has been reported to induce a cell cycle delay at the G^sub 2^/M phase. We tested the effects of cannabinoids on cell cycle progression in HeLa cells synchronized using a double-thymidine block-and-release protocol and found an increase in the population of G^sub 2^/M phase cells. We further found that CB1R activation augmented the interaction of 14-3-3[beta] with Wee1 and Cdc25B, and promoted phosphorylation of Cdc2 at Tyr-15. These results suggest that cannabinoids induce cell cycle delay at the G2/M phase by activating 14-3-3[beta].[PUBLICATION ABSTRACT]