Cancer letters, 2002-12, Vol.187 (1), p.143-151
2002
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Synergism between rViscumin and cisplatin is not dependent on ERCC-1 expression
- Ist Teil von
- Cancer letters, 2002-12, Vol.187 (1), p.143-151
- Ort / Verlag
- Shannon: Elsevier Ireland Ltd
- Erscheinungsjahr
- 2002
- Quelle
- Elsevier ScienceDirect Journals
- Beschreibungen/Notizen
- Interactions between recombinant mistletoe lectin (rViscumin) and anticancer drugs were investigated in vitro. rViscumin enhanced the cytotoxic effects of vincristine, mafosfamide, idarubicin and cisplatin in the human leukemia cell lines K562 and KG1a. In human marrow progenitor cells, rViscumin inhibited colony formation and did not exert any protective activity against cisplatin-induced inhibition of clonogenicity. Quantitative real-time reverse transription polymerase chain reaction analysis revealed that cisplatin treatment of K562 cells resulted in a 1.9-fold increase in mRNA expression of the nucleotide excision repair gene ERCC-1. This upregulation was not prevented when cells were post-incubated with rViscumin. Our study provides evidence that rViscumin is capable of enhancing cytotoxicity of anticancer agents in vitro. This synergism appears to be independent of transcriptional activity of DNA repair relevant genes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0304-3835eISSN: 1872-7980DOI: 10.1016/S0304-3835(02)00411-1Titel-ID: cdi_proquest_journals_1505362252
- Format
- –
- Schlagworte
- Adjuvants, Immunologic - pharmacology, Antineoplastic agents, Antineoplastic Agents - pharmacology, Apoptosis, Aqueous solutions, Base Pair Mismatch, Biological and medical sciences, Bone marrow, Bone Marrow - drug effects, Bone Marrow - metabolism, Cancer, Cancer therapies, Cell Division - drug effects, Cisplatin - pharmacology, Combined treatments (chemotherapy of immunotherapy associated with an other treatment), Cytotoxicity, DNA Repair, DNA-Binding Proteins, Drug interaction, Drug Synergism, Endonucleases, General pharmacology, Hematopoietic Stem Cells - drug effects, Hematopoietic Stem Cells - metabolism, Humans, Kinases, Medical sciences, Nucleotide excision repair, Pharmacognosy. Homeopathy. Health food, Pharmacology. Drug treatments, Plant Preparations - pharmacology, Plant Proteins, Proteins, Proteins - genetics, Proteins - metabolism, Real-time polymerase chain reaction, Recombinant mistletoe lectin, Reverse Transcriptase Polymerase Chain Reaction, Ribosome Inactivating Proteins, Type 2, RNA, Messenger - metabolism, RNA, Neoplasm, Toxins, Biological - pharmacology, Tumor Cells, Cultured - drug effects, Tumor Cells, Cultured - metabolism, Up-Regulation