Details

Autor(en) / Beteiligte

• Inagaki, S.(Kumamoto Univ. (Japan))
• Morimura, S
• Gondo, K
• Tang, Y
• Akutagawa, H
• Kida, K

Titel

Isolation of tryptophol as an apoptosis-inducing component of vinegar produced from boiled extract of black soybean [Glycine max] in human monoblastic leukemia U937 cells

Ist Teil von

• Bioscience, biotechnology, and biochemistry, 2007-02, Vol.71 (2), p.371-379

Ort / Verlag

Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• We isolated a novel apoptosis-inducing component, tryptophol, from vinegar produced from boiled extract of black soybean (black soybean vinegar). Compound-6 purified from an ethyl acetate extract of black soybean vinegar using high performance liquid chromatography (HPLC) induced fragmentation of DNA and the development of apoptotic bodies (characteristic physiological features of apoptosis) in U937 cells. By analysis of chemical structure, this active compound was identified as tryptophol. Tryptophol induced apoptosis involving caspase-8 and -3 activation, followed by cleavage of poly (ADP-ribose) polymerase (PARP), as shown by measurement of enzyme activity and immunoblot analysis. The cell viability of normal lymphocytes separated from human blood was less affected by tryptophol, and fragmentation of DNA was not induced in normal lymphocytes. These results indicate that tryptophol isolated from black soybean vinegar inhibited the proliferation of U937 cells by inducing apoptosis via a pathway involving caspase-8 followed by caspase-3, without affecting normal lymphocytes.