Bioscience, biotechnology, and biochemistry, 1999-03, Vol.63 (3), p.610-613
1999
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Details
- Autor(en) / Beteiligte
- Titel
- Autodegradation of protein disulfide isomerase
- Ist Teil von
- Bioscience, biotechnology, and biochemistry, 1999-03, Vol.63 (3), p.610-613
- Ort / Verlag
- Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Erscheinungsjahr
- 1999
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Protein disulfide isomerase (PDI) and its degradation products were found in HepG2, COS-1, and CHO-K1 cells. Whether or not the products were formed through autodegradation of PDI was examined, since PDI contains the CGHC motif, which is the active center of proteolytic activity in ER-60 protease. Commercial bovine PDI was autodegraded to produce a trimmed PDI. In addition, human recombinant PDI also had autodegradation activity. Mutant recombinant PDIs with CGHC motifs of which cysteins residues were replaced with serine or alanine residues were prepared. However, they were not autodegraded, suggesting the cysteine residues of motifs are necessary for autodegradation
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0916-8451eISSN: 1347-6947DOI: 10.1271/bbb.63.610Titel-ID: cdi_proquest_journals_1449435733
- Format
- –
- Schlagworte
- Analytical, structural and metabolic biochemistry, Animals, Biological and medical sciences, Cattle, Cell Line, CHO Cells, COS Cells, Cricetinae, DEGRADACION, DEGRADATION, ENDOPLASMIC RETICULUM, Endoplasmic Reticulum, Smooth - enzymology, Enzymes and enzyme inhibitors, Fundamental and applied biological sciences. Psychology, Humans, ISOMERASAS, ISOMERASE, ISOMERASES, Mice, Mutagenesis, Site-Directed, protein disulfide isomerase, Protein Disulfide-Isomerases - biosynthesis, Protein Disulfide-Isomerases - genetics, Protein Disulfide-Isomerases - metabolism, PROTEINAS, PROTEINE, PROTEINS, proteolytic activity, Recombinant Proteins - biosynthesis, Recombinant Proteins - isolation & purification, RETICULO ENDOPLASMATICO, RETICULUM ENDOPLASMIQUE