Details

Autor(en) / Beteiligte

• YAMAUCHI, Tomohiro
• INAGAKI, Taisuke
• OHISHI, Nobuko
• YAGI, Kunio

Titel

Rapid Preparation of Subfractions Density Lipoprotein from Hypercholesterolemic Rabbit Serum

Ist Teil von

• Journal of Clinical Biochemistry and Nutrition, 1990, Vol.8(1), pp.9-19

Ort / Verlag

Gifu: SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Erscheinungsjahr

1990

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• A method for rapid preparation of subfractions of low density lipoprotein (LDL) from the serum of cholesterol-fed rabbits was devised. The method includes three steps: (1) removal of chylomicrons and very low density lipoprotein by ultracentrifugation with a vertical rotor for 1h; (2) separation of LDL subfractions, LDL-1, LDL-2, and LDL-3, by ultracentrifugation with a vertical rotor for 2h; and (3) Fast Protein, Polypeptide, Polynucleotide Liquid Chromatography (FPLC) for purification of these LDL subfractions. The whole procedure of the present method could be completed within 13h. On the basis of size and density distribution, LDL-1 corresponded to intermediate density lipoprotein obtained by the standard sequential floatation method (d=1.006-1.019) from the same serum. Gel electrophoresis, density distribution, and FPLC analysis showed that LDL-1 was contaminated with neither other lipoproteins nor serum proteins. LDL-3 was found to contain LDL obtained by the previously reported method from the serum of a rabbit fed a normal diet. LDL-2 had properties intermediate between those of LDL-1 and LDL-3.