Details

Autor(en) / Beteiligte

• TAN, JIALI
• WANG, CONGYING
• XIANG, BIN
• HAN, RUIHONG
• GUO, ZHENFEI

Titel

Hydrogen peroxide and nitric oxide mediated cold‐ and dehydration‐induced myo‐inositol phosphate synthase that confers multiple resistances to abiotic stresses

Ist Teil von

• Plant, cell and environment, 2013-02, Vol.36 (2), p.288-299

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2013

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• ABSTRACT myo‐Inositol phosphate synthase (MIPS) is the key enzyme of myo‐inositol synthesis, which is a central molecule required for cell metabolism and plant growth as a precursor to a large variety of compounds. A full‐length fragment of MfMIPS1 cDNA was cloned from Medicago falcata that is more cold‐tolerant than Medicago sativa. While MfMIPS1 transcript was induced in response to cold, dehydration and salt stress, MIPS transcript and myo‐inositol were maintained longer and at a higher level in M. falcata than in M. sativa during cold acclimation at 5 °C. MfMIPS1 transcript was induced by hydrogen peroxide (H2O2) and nitric oxide (NO), but was not responsive to abscisic acid (ABA). Pharmacological experiments revealed that H2O2 and NO are involved in the regulation of MfMIPS1 expression by cold and dehydration, but not by salt. Overexpression of MfMIPS1 in tobacco increased the MIPS activity and levels of myo‐inositol, galactinol and raffinose, resulting in enhanced resistance to chilling, drought and salt stresses in transgenic tobacco plants. It is suggested that MfMIPS1 is induced by diverse environmental factors and confers resistance to various abiotic stresses. MfMIPS1 transcript was induced in response to cold, dehydration and salt stress, while MIPS transcript and myo‐inositol were maintained longer and at higher level in M. falcata than in M. sativa during cold acclimation. MfMIPS1 transcript was induced by hydrogen peroxide (H2O2) and nitric oxide (NO), but not responsive to abscisic acid (ABA). H2O2 and NO are involved in regulation of MfMIPS1 expression by cold and dehydration, but not by salt. Overexpression of MfMIPS1 in tobacco increased MIPS activity and levels of myo‐inositol, galactinol, and raffinose, resulting in enhanced resistance to chilling, drought, and salt stresses in transgenic tobacco plants.