Details

Autor(en) / Beteiligte

• Chodok, Pichit
• Eiamsa-ard, Pradinunt
• Cove, David J
• Quatrano, Ralph S
• Kaewsuwan, Sireewan

Titel

Identification and functional characterization of two [Delta]^sup 12^-fatty acid desaturases associated with essential linoleic acid biosynthesis in Physcomitrella patens

Ist Teil von

• Journal of industrial microbiology & biotechnology, 2013-08, Vol.40 (8), p.901

Ort / Verlag

Fairfax: Springer Nature B.V

Erscheinungsjahr

2013

Quelle

Springer journals

Beschreibungen/Notizen

• Two [Delta]^sup 12^-desaturases associated with the primary steps of long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis were successfully cloned from Physcomitrella patens and their functions identified. The open reading frames (ORFs) of PpFAD2-1 and PpFAD2-2 consisted of 1,128 bp and code for 375 amino acids. Their deduced polypeptides showed 62-64 % identity to microsomal [Delta]^sup 12^-desaturases from other higher plants, and each contained the three histidine clusters typical of the catalytic domains of such enzymes. Yeast cells transformed with plasmid constructs containing PpFAD2-1 or PpFAD2-2 produced an appreciable amount of hexadecadienoic (16:2 [Delta]^sup 9,12^) and linoleic acids (18:2 [Delta]^sup 9,12^), not normally present in wild-type yeast cells, indicating that the genes encoded functional [Delta]^sup 12^-desaturase enzymes. In addition, reduction of the growth temperature from 30 to 15 °C resulted in increased accumulation of unsaturated fatty acid products.[PUBLICATION ABSTRACT]