Details

Autor(en) / Beteiligte

• Zhong, Weiting
• Chi, Gefu
• Jiang, Lanxiang
• Soromou, Lanan Wassy
• Chen, Na
• Huo, Meixia
• Guo, Weixiao
• Deng, Xuming
• Feng, Haihua

Titel

p-Cymene Modulates In Vitro and In Vivo Cytokine Production by Inhibiting MAPK and NF-[kappa]B Activation

Ist Teil von

• Inflammation, 2013-06, Vol.36 (3), p.529

Ort / Verlag

New York: Springer Nature B.V

Erscheinungsjahr

2013

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The present study was designed to investigate the effects of p-cymene on lipopolysaccharide (LPS)-induced inflammatory cytokine production both in vitro and in vivo. The production of tumor necrosis factor-[alpha] (TNF-[alpha]), interleukin-1[beta] (IL-1[beta]), interleukin-6 (IL-6), and interleukin-10 (IL-10) in LPS-stimulated RAW 264.7 cells and C57BL/6 mice was evaluated by sandwich ELISA. Meanwhile, the mRNA levels of cytokine genes were examined in vitro by semiquantitative RT-PCR. In a further study, we analyzed the activation of nuclear factor-[kappa]B (NF-[kappa]B) and mitogen-activated protein kinase (MAPK) signaling pathways by western blotting. We found that p-cymene significantly regulated TNF-[alpha], IL-1[beta], and IL-6 production in LPS-stimulated RAW 264.7 cells. Furthermore, the levels of relative mRNAs were also found to be downregulated. In in vivo trail, p-cymene markedly suppressed the production of TNF-[alpha] and IL-1[beta] and increased IL-10 secretion. We also found that p-cymene inhibited LPS-induced activation of extracellular signal receptor-activated kinase 1/2, p38, c-Jun N-terminal kinase, and I[kappa]B[alpha]. These results suggest that p-cymene may have a potential anti-inflammatory action on cytokine production by blocking NF-[kappa]B and MAPK signaling pathways.[PUBLICATION ABSTRACT] Erratum DOI: 10.1007/s10753-013-9618-y