Details

Autor(en) / Beteiligte

• Hobson, Neil
• Deyholos, Michael K

Titel

LuFLA1^sub PRO^ and LuBGAL1^sub PRO^ promote gene expression in the phloem fibres of flax (Linum usitatissimum)

Ist Teil von

• Plant cell reports, 2013-04, Vol.32 (4), p.517

Ort / Verlag

Berlin: Springer Nature B.V

Erscheinungsjahr

2013

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Cell type-specific promoters were identified that drive gene expression in an industrially important product. To identify flax (Linum usitatissimum) gene promoters, we analyzed the genomic regions upstream of a fasciclin-like arabinogalactan protein (LuFLA1) and a beta-galactosidase (LuBGAL1). Both of these genes encode transcripts that have been found to be highly enriched in tissues bearing phloem fibres. Using a beta-glucuronidase (GUS) reporter construct, we found that a 908-bp genomic sequence upstream of LuFLA1 (LuFLA1^sub PRO^) directed GUS expression with high specificity to phloem fibres undergoing secondary cell wall development. The DNA sequence upstream of LuBGAL1 (LuBGAL1^sub PRO^) likewise produced GUS staining in phloem fibres with developing secondary walls, as well as in tissues of developing flowers and seed bolls. These data provide further evidence of a specific role for LuFLA1 in phloem fibre development, and demonstrate the utility of LuFLA1^sub PRO^ and LuBGAL1^sub PRO^ as tools for biotechnology and further investigations of phloem fibre development.[PUBLICATION ABSTRACT]