Details

Autor(en) / Beteiligte

• YAMAUCHI, N
• NISHIKAWA, S
• KIDO, J.-I
• OHISHI, K
• NAGATA, T
• KATAOKA, M
• SHINOHARA, H
• ISHIDA, H

Titel

Relationship between the expression of parathyroid hormone receptors and hormonal effect during rat osteoprogenitor cell differentiation

Ist Teil von

• Journal of bone and mineral metabolism, 1997-03, Vol.15 (1), p.17-22

Ort / Verlag

Tokyo: Springer

Erscheinungsjahr

1997

Quelle

SpringerLINK Contemporary (Konsortium Baden-Württemberg)

Beschreibungen/Notizen

• The number of parathyroid hormone (PTH) receptors in rat calvaria (RC) cells increased with culture duration, reached a maximum on day 13 when the cells had not yet differentiated into osteoblasts, and then gradually decreased by day 21. However, short exposure (48h) of RC cells to PTH-(1-34) before day 12, but not after day 15, did not decrease alkaline phosphatase (ALP) activity of the cells. Bone-like nodule (BN) formation was also suppressed only when PTH-(1-34) was present during the late culture period (days 14-21). Pretreating the cells with staurosporine, a protein kinase C inhibitor, on day 7 resulted in augmentation of PTH-(1-34)-induced cyclic adenosine monophosphate (cAMP) accumulation and inhibition of ALP activity. PTH-(7-34), which only activates PKC, however, had no effect on RC cell differentiation under any conditions used.[PUBLICATION ABSTRACT]