Details

Autor(en) / Beteiligte

• Zhao, Jun-Qiong
• Xie, Si-Si
• Liu, Wen-Bin
• Xiao, Ya-Mei
• Zeng, Xiao-Ming
• Deng, Mi
• Gong, Lili
• Liu, Jin-Ping
• Chen, Pei-Chao
• Zhou, Jie
• Hu, Xiao-Hui
• Lv, Jia-Han
• Yu, Xiang-Qian
• Wang, Dao
• Li, Chi
• Peng, Yun-Lei
• Liao, Gao-Peng
• Liu, Yun
• Li, David Wan-Cheng

Titel

Molecular Cloning of the Genes Encoding the PR55/B[beta]/[delta] Regulatory Subunits for PP-2A and Analysis of Their Functions in Regulating Development of Goldfish, Carassius auratus

Ist Teil von

• Gene regulation and systems biology, 2010-01, Vol.4, p.135

Ort / Verlag

Thousand Oaks, CA: Sage Publications Ltd

Erscheinungsjahr

2010

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The protein phosphatase-2A (PP-2A), one of the major phosphatases in eukaryotes, is a heterotrimer, consisting of a scaffold A subunit, a catalytic C subunit and a regulatory B subunit. Previous studies have shown that besides regulating specific PP-2A activity, various B subunits encoded by more than 16 different genes, may have other functions. To explore the possible roles of the regulatory subunits of PP-2A in vertebrate development, we have cloned the PR55/B family regulatory subunits: β and δ, analyzed their tissue specific and developmental expression patterns in Goldfish (Carassius auratus). Our results revealed that the full-length cDNA for PR55/Bβ consists of 1940 bp with an open reading frame of 1332 nucleotides coding for a deduced protein of 443 amino acids. The full length PR55/Bδ cDNA is 2163 bp containing an open reading frame of 1347 nucleotides encoding a deduced protein of 448 amino acids. The two isoforms of PR55/B display high levels of sequence identity with their counterparts in other species. The PR55/Bβ mRNA and protein are detected in brain and heart. In contrast, the PR55/Bδ is expressed in all 9 tissues examined at both mRNA and protein levels. During development of goldfish, the mRNAs for PR55/Bβ and PR55/Bδ show distinct patterns. At the protein level, PR55/Bδ is expressed at all developmental stages examined, suggesting its important role in regulating goldfish development. Expression of the PR55/Bδ anti-sense RNA leads to significant downregulation of PR55/Bδ proteins and caused severe abnormality in goldfish trunk and eye development. Together, our results suggested that PR55/Bδ plays an important role in governing normal trunk and eye formation during goldfish development.