Details

Autor(en) / Beteiligte

• Cui, Lan Hai

Titel

Construction of Plant Expression Vector with rip Gene of Barley and its Transferring into Rice

Ort / Verlag

ProQuest Dissertations & Theses

Erscheinungsjahr

2003

Quelle

ProQuest Dissertations & Theses A&I

Beschreibungen/Notizen

• Rice is one of the most important crops, but the yield is declined every year because of its fungi disease. One of the common protecting method is chemical medicine. Although it has some effect, it also brings some disadvantages, such as heavy polluted environment, maladjustment ecology and harm to human healthy. It is testify that culture the fungi-resistant cultivates is the most economic and available way. However, the conventional breeding can't meet the need of cultivating the fungi-resistant varieties, because of its long period, short fungi-resistant genes and disappearing resistance with the changes of pathogen. So, using the genetic engineering to breed the fungi-resistant varieties is a available method. In this study, we constructed two plant expression vector with rip gene from barley and linked to two promoters which have high promoting efficiency in monocotyledonous plants, respectively, and then transferred to rice mediated by Agrobacterium Tumefaciens. The main factors of affecting transformat