Details

Autor(en) / Beteiligte

• Aggarwal, Shalini
• Banerjee, Nirjhar
• Parihari, Shashwati
• Roy, Jyotirmoy
• Bojak, Kharanshu
• Shah, Rhythm
• Srivastava, Sanjeeva

Titel

Metabolomics: Role in pathobiology and therapeutics of COVID-19

Ist Teil von

• Multi-Pronged Omics Technologies to Understand COVID-19, 2023, p.75-99

Auflage

1

Ort / Verlag

United Kingdom: CRC Press

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Metabolites are small biomolecules that play a pivotal role in the physiological pathways in a living organism. Studying metabolome profiles can thus reflect the physiochemical state of an individual. Comparative metabolomics study can illustrate potential biomarkers of diagnosis and prognosis, pave ways for therapeutic strategies, and identify effects of various external chemical exposures. Metabolome analysis workflow includes sample collection, viral inactivation, metabolite extraction, statistical analysis, identification, and data interpretation. An extension of the metabolomics study to exposomes gives an insight of how the external environment can serve as potential risk for various diseases. The analytical platforms such as mass spectrometry (MS), nuclear magnetic resonance spectrometry (NMR), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy permit detection and identification of a wide range of metabolites. Various freely available systems, such as Galaxy, XCMS, Compound Discoverer, Mummichog, MetaboAnalyst, and others, can be used to identify compounds and subsequently assess their statistical significance from mass spectrometry-generated files. Recent advances in high-resolution techniques for biomolecule segregation have resulted in the identification of critical metabolites affected in diseases such as COVID-19. Metabolites being highly dynamic do not yield a postinfection trail, reducing the chances of false positives and making them desirable biomarker candidates. Earlier studies reported that the alteration of amino acid, fatty acid, carbon metabolism, and nucleic acid metabolism could potentially result in severe clinical manifestations involving multiple organ failures leading to the patient's death. Furthermore, metabolomics can indicate the exposomics, i.e., the study of environmental stress and exposures effecting upon the human health.