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The examination of aspirated material for the diagnosis of parasitic infections is useful when routine specimens and methods have failed to demonstrate the organisms. Aspirates most commonly processed in the parasitology laboratory include fine‐needle aspirates and duodenal aspirates. Fluid specimens collected by bronchoscopy include bronchoalveolar lavage fluids and bronchial washings. Biopsy specimens are recommended for the diagnosis of tissue parasites. The procedures that may be used for this purpose in addition to standard histological preparations are impression smears and teased and squash preparations of biopsy tissue from skin, muscle, cornea, intestine, liver, lung, and brain. The diagnosis of larval cestode infections often depends on the use of invasive procedures and subsequent examination of aspirate or biopsy specimens. Although slides prepared from these specimens can be stained using a number of approaches, use of the modified trichrome‐Ryan blue technique provides excellent results using routine light microscopy for the identification of cestode hooklets.