Details

Autor(en) / Beteiligte

• Scolnick, E.M.
• Oliff, A.
• Linemeyer, D.
• Ruscetti, S.
• Testa, N.G.
• Dexter, T.M.
• Fields, Bernard N
• Jaenisch, Rudolf

Titel

MOLECULAR GENETICS AND CELL CULTURE ASSAYS FOR HELPER-INDEPENDENT AND REPLICATION-DEFECTIVE COMPONENTS OF THE FRIEND VIRUS COMPLEX

Ist Teil von

• Animal Virus Genetics, 1980, p.425-441

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1980

Beschreibungen/Notizen

• From the Friend virus complex, the helper-independent component, F-MuLV, and the replication-defective component, SFFV, have been biologically cloned, and subsequently molecularly cloned in bacterial vectors. The molecularly cloned forms of each virus are infectious in gene transfer studies. F-MuLV induces an erythropoietic proliferative disease with anemia in newborn Swiss mice, while SFFV determines a polycythemic erythroblastosis in adult mice. By molecular cloning techniques, we have identified that the 3′ 3.1 Kb of F-MuLV carries the disease-inducing gene(s) of F-MuLV. Cell culture conditions have been developed which allow long-term erythropoiesis in Dexter bone-marrow cultures, and effects of F-MuLV and of SFFV have been observed in this culture system. The results suggest for the first time a cell culture assay for the growth promoting effects on hematopoietic cells of helper-independent type-C leukemia viruses.