Details

Autor(en) / Beteiligte

• Irving, Phil
• Troxler, Laurent
• Heuer, Timothy S.
• Belvin, Marcia
• Kopczynski, Casey
• Reichhart, Jean-Marc
• Hoffmann, Jules A.
• Hetru, Charles

Titel

A Genome-Wide Analysis of Immune Responses in Drosophila

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2001-12, Vol.98 (26), p.15119-15124

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2001

Quelle

MEDLINE

Beschreibungen/Notizen

• Oligonucleotide DNA microarrays were used for a genome-wide analysis of immune-challenged Drosophila infected with Gram-positive or Gram-negative bacteria, or with fungi. Aside from the expression of an established set of immune defense genes, a significant number of previously unseen immune-induced genes were found. Genes of particular interest include corin- and Stubble-like genes, both of which have a type II transmembrane domain; easter- and snake-like genes, which may fulfil the roles of easter and snake in the Toll pathway; and a masquerade-like gene, potentially involved in enzyme regulation. The microarray data has also helped to greatly reduce the number of target genes in large gene groups, such as the proteases, helping to direct the choices for future mutant studies. Many of the up-regulated genes fit into the current conceptual framework of host defense, whereas others, including the substantial number of genes with unknown functions, offer new avenues for research.