Details

Autor(en) / Beteiligte

• Wang, Ruobing
• Epstein, Judith
• Baraceros, Fe Maria
• Gorak, Edward J.
• Charoenvit, Yupin
• Carucci, Daniel J.
• Hedstrom, Richard C.
• Rahardjo, Nancy
• Gay, Tanya
• Hobart, Peter
• Stout, Rick
• Jones, Trevor R.
• Richie, Thomas L.
• Parker, Suezanne E.
• Doolan, Denise L.
• Norman, Jon
• Hoffman, Stephen L.

Titel

Induction of CD4+T Cell-Dependent CD8+Type 1 Responses in Humans by a Malaria DNA Vaccine

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2001-09, Vol.98 (19), p.10817-10822

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2001

Quelle

MEDLINE

Beschreibungen/Notizen

• We assessed immunogenicity of a malaria DNA vaccine administered by needle i.m. or needleless jet injection [i.m. or i.m./intradermally (i.d.)] in 14 volunteers. Antigen-specific IFN-γ responses were detected by enzyme-linked immunospot (ELISPOT) assays in all subjects to multiple 9- to 23-aa peptides containing class I and/or class II restricted epitopes, and were dependent on both CD8+and CD4+T cells. Overall, frequency of response was significantly greater after i.m. jet injection. CD8+-dependent cytotoxic T lymphocytes (CTL) were detected in 8/14 volunteers. Demonstration in humans of elicitation of the class I restricted IFN-γ responses we believe necessary for protection against the liver stage of malaria parasites brings us closer to an effective malaria vaccine.