Proceedings of the National Academy of Sciences - PNAS, 1999-03, Vol.96 (5), p.2159-2164
1999
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Details
- Autor(en) / Beteiligte
- Titel
- In vitro Hematopoietic and Endothelial Potential of flk-1-/-Embryonic Stem Cells and Embryos
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1999-03, Vol.96 (5), p.2159-2164
- Ort / Verlag
- United States: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1999
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Mice deficient in the Flk-1 receptor tyrosine kinase are known to die in utero because of defective vascular and hematopoietic development. Here, we show that flk-1-/-embryonic stem cells are nevertheless able to differentiate into hematopoietic and endothelial cells in vitro, although they give rise to a greatly reduced number of blast colonies, a measure of hemangioblast potential. Furthermore, normal numbers of hematopoietic progenitors are found in 7.5-day postcoitum flk-1-/-embryos, even though 8.5-day postcoitum flk-1-/-embryos are known to be deficient in such cells. Our results suggest that hematopoietic/endothelial progenitors arise independently of Flk-1, but that their subsequent migration and expansion require a Flk-1-mediated signal.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.96.5.2159Titel-ID: cdi_pnas_primary_96_5_2159
- Format
- –
- Schlagworte
- Animals, Biological Sciences, Blasts, Cell Adhesion, Cell growth, Cell lines, Cells, Cells, Cultured, Cellular differentiation, Embryo, Mammalian, Embryonic stem cells, Embryos, Endothelial cells, Endothelium, Vascular - cytology, Endothelium, Vascular - physiology, Flow Cytometry, Gene Expression Regulation, Developmental, Hematopoietic stem cells, Hematopoietic Stem Cells - cytology, Hematopoietic Stem Cells - physiology, Laboratory animals, Mice, Mice, Knockout, Progenitor cells, Receptor Protein-Tyrosine Kinases - deficiency, Receptor Protein-Tyrosine Kinases - genetics, Receptor Protein-Tyrosine Kinases - physiology, Receptors, Growth Factor - deficiency, Receptors, Growth Factor - genetics, Receptors, Growth Factor - physiology, Receptors, Mitogen - physiology, Receptors, Vascular Endothelial Growth Factor, Reverse Transcriptase Polymerase Chain Reaction, Rodents, Signal Transduction, Stem cells, Stem Cells - physiology