Details

Autor(en) / Beteiligte

• Landry, L.G. (Monmouth University, West Long Branch, NJ.)
• Stapleton, A.E
• Lim, J
• Hoffman, P
• Hays, J.B
• Walbot, V
• Last, R.L

Titel

An Arabidopsis photolyase mutant is hypersensitive to ultraviolet-B radiation

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1997-01, Vol.94 (1), p.328-332

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• Photolyases are DNA repair enzymes that use energy from blue light to repair pyrimidine dimers. We report the isolation of an Arabidopsis thaliana mutant (uvr2-1) that is defective in photorepair of cyclobutylpyrimidine dimers (CPDs). Whereas uvr2-1 is indistinguishable from wild type in the absence of UV light, low UV-B levels inhibit growth and cause leaf necrosis. uvr2-1 is more sensitive to UV-B than wild type when placed under white light after UV-B treatment. In contrast, recovery in darkness or in light lacking photoreactivating blue light results in equal injury in uvr2-1 and wild type. The uvr2-1 mutant is unable to remove CPDs in vivo, and plant extracts lack detectable photolyase activity. This recessive mutation segregates as a single gene located near the top of chromosome 1, and is a structural gene mutation in the type II CPD photolyase PHR1. This mutant provides evidence that CPD photolyase is required for plant survival in the presence of UV-B light