Details

Autor(en) / Beteiligte

• Sedlak, Thomas W.
• Oltvai, Zoltan N.
• Yang, Elizabeth
• Wang, Kun
• Boise, Lawrence H.
• Thompson, Craig B.
• Korsmeyer, Stanley J.

Titel

Multiple Bcl-2 Family Members Demonstrate Selective Dimerizations with Bax

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1995-08, Vol.92 (17), p.7834-7838

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

1995

Quelle

MEDLINE

Beschreibungen/Notizen

• A family of Bcl-2-related proteins regulates cell death and shares highly conserved BH1 and BH2 domains. BH1 and BH2 domains of Bcl-2 were required for it to heterodimerize with Bax and to repress apoptosis. A yeast two-hybrid assay accurately reproduced this interaction and defined a selectivity and hierarchy of further dimerizations. Bax also heterodimerizes with Bcl-XL, Mcl-1, and A1. A Gly-159 → Ala substitution in BH1 of Bcl-xLdisrupted its heterodimerization with Bax and abrogated its inhibition of apoptosis in mammalian cells. This suggests that the susceptibility to apoptosis is determined by multiple competing dimerizations in which Bax may be a common partner.