Proceedings of the National Academy of Sciences - PNAS, 1995-07, Vol.92 (14), p.6374-6378
1995
Details
- Autor(en) / Beteiligte
- Titel
- Activation of Mitogen-Activated Protein Kinases by Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor in Capillary Endothelial Cells is Inhibited by the Antiangiogenic Factor 16-kDa N-Terminal Fragment of Prolactin
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1995-07, Vol.92 (14), p.6374-6378
- Ort / Verlag
- United States: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1995
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A number of factors both stimulating and inhibiting angiogenesis have been described. In the current work, we demonstrate that the angiogenic factor vascular endothelial growth factor (VEGF) activates mitogen-activated protein kinase (MAPK) as has been previously shown for basic fibroblast growth factor. The antiangiogenic factor 16-kDa N-terminal fragment of human prolactin inhibits activation of MAPK distal to autophosphorylation of the putative VEGF receptor, Flk-1, and phospholipase C-γ. These data show that activation and inhibition of MAPK may play a central role in the control of angiogenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424, 1091-6490eISSN: 1091-6490DOI: 10.1073/pnas.92.14.6374Titel-ID: cdi_pnas_primary_92_14_6374_fulltext
- Format
- –
- Schlagworte
- Angiogenesis, Animals, Antibodies, Biochemistry, biophysics & molecular biology, Biochimie, biophysique & biologie moléculaire, Blotting, Western, Ca(2+)-Calmodulin Dependent Protein Kinase/metabolism, Calcium-Calmodulin-Dependent Protein Kinases, Calcium-Calmodulin-Dependent Protein Kinases - metabolism, Capillaries, Cattle, Cell culture techniques, Cells, Cultured, Cellular biology, Cerebrovascular Circulation, Electrophoresis, Polyacrylamide Gel, Endocrinology, Endothelial cells, Endothelial Growth Factors, Endothelial Growth Factors - pharmacology, Endothelium, Vascular, Endothelium, Vascular - enzymology, Enzyme Activation, Fibroblast Growth Factor 2, Fibroblast Growth Factor 2 - pharmacology, Gels, Humans, Life Sciences, Lymphokines, Lymphokines - pharmacology, Molecular Weight, Peptide Fragments, Peptide Fragments - pharmacology, Phosphoproteins, Phosphoproteins - isolation & purification, Phosphoproteins - metabolism, Phosphoproteins/isolation & purification/metabolism, Phosphorylation, Phosphotyrosine, Prolactin, Prolactin - pharmacology, Proteins, Receptors, Recombinant Proteins, Recombinant Proteins - pharmacology, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S, Sciences du vivant, Tyrosine, Tyrosine - analogs & derivatives, Tyrosine - analysis, Tyrosine/analogs & derivatives/analysis, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors