Proceedings of the National Academy of Sciences - PNAS, 1993-04, Vol.90 (7), p.2812-2816
1993
Details
- Autor(en) / Beteiligte
- Titel
- Adenovirus-Mediated Transfer of Low Density Lipoprotein Receptor Gene Acutely Accelerates Cholesterol Clearance in Normal Mice
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1993-04, Vol.90 (7), p.2812-2816
- Ort / Verlag
- Washington, DC: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1993
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We have explored the use of adenovirus-mediated gene transfer to transiently elicit production of low density lipoprotein (LDL) receptors in mice. A recombinant adenovirus carrying the human LDL receptor cDNA restored LDL receptor function in receptor-deficient cultured cells. Intravenous injection of recombinant virus acutely lowered plasma cholesterol levels and increased the rate of125I-labeled LDL clearance from the circulation in normal mice. At 4 days after virus injection, the t1/2of plasma LDL was reduced up to 10-fold. An estimated 90% of the parenchymal cells in liver expressed the adenovirus-transferred genes as judged by immunofluorescence of LDL receptors or by β-galactosidase staining. These results demonstrate that adenovirus-mediated transfer of the LDL receptor gene provides an efficient way of augmenting LDL receptor gene function in the liver over the short term.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.90.7.2812Titel-ID: cdi_pnas_primary_90_7_2812
- Format
- –
- Schlagworte
- Adenoviridae - genetics, adenovirus, Adenoviruses, Animals, beta-Galactosidase - genetics, beta-Galactosidase - metabolism, Biological and medical sciences, Blood plasma, CHO Cells, Cholesterol, Cholesterol - metabolism, Cholesterols, Cricetinae, Cultured cells, General and cellular metabolism. Vitamins, Genetics, Humans, Intravenous injections, LDL receptors, Lipids, Lipoproteins, LDL - blood, Lipoproteins, LDL - metabolism, Lipoproteins, LDL - pharmacokinetics, Liver, Luciferases - genetics, Luciferases - metabolism, Medical sciences, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Organ Specificity, Pharmacology. Drug treatments, Receptors, LDL - genetics, Receptors, LDL - metabolism, Recombinant Fusion Proteins - metabolism, Rodents, Tissue Distribution, Transfection, Viruses