Details

Autor(en) / Beteiligte

• Dohmen, R.J. (Massachusetts Institute of Technology, Cambridge, MA)
• Madura, K
• Bartel, B
• Varshavsky, A

Titel

The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1991-08, Vol.88 (16), p.7351-7355

Ort / Verlag

Washington, DC: National Academy of Sciences of the United States of America

Erscheinungsjahr

1991

Quelle

MEDLINE

Beschreibungen/Notizen

• The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all organisms examined, from mammals to bacteria. We show that UBC2(RAD6), one of at least seven ubiquitin-conjugating enzymes in the yeast Saccharomyces cerevisiae, is essential for multiubiquitination and degradation of the N-end rule substrates. We also show that UBC2 is physically associated with UBR1, the recognition component of the N-end rule pathway. These results indicate that some of the UBC2 functions, which include DNA repair, induced mutagenesis, sporulation, and regulation of retrotransposition, are mediated by protein degradation via the N-end rule pathway.