Proceedings of the National Academy of Sciences - PNAS, 1972-10, Vol.69 (10), p.2950-2953
1972
Details
- Autor(en) / Beteiligte
- Titel
- Assembly of Tobacco Mosaic Virus in vitro: Effect of State of Polymerization of the Protein Component
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1972-10, Vol.69 (10), p.2950-2953
- Ort / Verlag
- United States: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1972
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- We previously proposed that the assembly of tobacco mosaic virus is initiated by disks of 34 protein subunits attaching to the RNA, after which the particle grows by the addition of further disks. Other workers have reported growth from ``A-protein'' instead. We now report experiments that confirm our previous results and show that the contrary findings are largely due to a nonequilibrium form of A-protein that has a ``memory'' for the disk state and rapidly reaggregates, either in solution or on the growing particle.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.69.10.2950Titel-ID: cdi_pnas_primary_69_10_2950
- Format
- –
- Schlagworte
- Aggregation, Biological Sciences: Biochemistry, Diphosphates, Electron microscopy, Hydrogen-Ion Concentration, Kinetics, Light scattering, Macromolecular Substances, Nucleoproteins, Nucleoproteins - biosynthesis, Peptide Chain Initiation, Translational, Phosphates, Protein Conformation, Protein subunits, RNA, RNA, Viral - metabolism, Temperature, Tobacco mosaic virus, Tobacco Mosaic Virus - metabolism, Viral Proteins - metabolism