Details

Autor(en) / Beteiligte

• Gaedicke, Simone
• Braun, Friederike
• Prasad, Shruthi
• Machein, Marcia
• Firat, Elke
• Hettich, Michael
• Gudihal, Ravindra
• Zhu, Xuekai
• Klingner, Kerstin
• Schüler, Julia
• Herold-Mende, Christel C
• Grosu, Anca-Ligia
• Behe, Martin
• Weber, Wolfgang
• Mäcke, Helmut
• Niedermann, Gabriele

Titel

Noninvasive positron emission tomography and fluorescence imaging of CD133⁺ tumor stem cells

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2014-02, Vol.111 (6), p.E692-E701

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection of AC133 ⁺ tumor stem cells by PET and near-infrared fluorescence molecular tomography in subcutaneous and orthotopic glioma xenografts using antibody-based tracers. Particularly, microPET with ⁶⁴Cu-NOTA-AC133 mAb yielded high-quality images with outstanding tumor-to-background contrast, clearly delineating subcutaneous tumor stem cell-derived xenografts from surrounding tissues. Intracerebral tumors as small as 2–3 mm also were clearly discernible, and the microPET images reflected the invasive growth pattern of orthotopic cancer stem cell-derived tumors with low density of AC133 ⁺ cells. These data provide a basis for further preclinical and clinical use of the developed tracers for high-sensitivity and high-resolution monitoring of AC133 ⁺ tumor stem cells.