Proceedings of the National Academy of Sciences - PNAS, 2014-07, Vol.111 (29), p.10580-10585
2014
Details
- Autor(en) / Beteiligte
- Titel
- uncoupling channel within the c-subunit ring of the F₁FO ATP synthase is the mitochondrial permeability transition pore
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2014-07, Vol.111 (29), p.10580-10585
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Mitochondria maintain tight regulation of inner mitochondrial membrane (IMM) permeability to sustain ATP production. Stressful events cause cellular calcium (Ca ²⁺) dysregulation followed by rapid loss of IMM potential known as permeability transition (PT), which produces osmotic shifts, metabolic dysfunction, and cell death. The molecular identity of the mitochondrial PT pore (mPTP) was previously unknown. We show that the purified reconstituted c-subunit ring of the F O of the F ₁F O ATP synthase forms a voltage-sensitive channel, the persistent opening of which leads to rapid and uncontrolled depolarization of the IMM in cells. Prolonged high matrix Ca ²⁺ enlarges the c-subunit ring and unhooks it from cyclophilin D/cyclosporine A binding sites in the ATP synthase F ₁, providing a mechanism for mPTP opening. In contrast, recombinant F ₁ beta-subunit applied exogenously to the purified c-subunit enhances the probability of pore closure. Depletion of the c-subunit attenuates Ca ²⁺-induced IMM depolarization and inhibits Ca ²⁺ and reactive oxygen species-induced cell death whereas increasing the expression or single-channel conductance of the c-subunit sensitizes to death. We conclude that a highly regulated c-subunit leak channel is a candidate for the mPTP. Beyond cell death, these findings also imply that increasing the probability of c-subunit channel closure in a healthy cell will enhance IMM coupling and increase cellular metabolic efficiency.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.1401591111Titel-ID: cdi_pnas_primary_111_29_10580
- Format
- –
- Schlagworte
- Animals, Biological Sciences, Calcium - pharmacology, Cell Death - drug effects, HEK293 Cells, Humans, Ion Channel Gating - drug effects, Ion Channels - metabolism, Liposomes - metabolism, Mitochondria - drug effects, Mitochondria - metabolism, Mitochondrial Membrane Transport Proteins - chemistry, Mitochondrial Membrane Transport Proteins - metabolism, Mitochondrial Membranes - drug effects, Mitochondrial Membranes - metabolism, Mutation - genetics, Protein Conformation, Protein Subunits - metabolism, Proton-Translocating ATPases - chemistry, Proton-Translocating ATPases - metabolism, Rats, Reactive Oxygen Species - metabolism