Details

Autor(en) / Beteiligte

• Gonzalez, Federico
• Barragan Monasterio, Montserrat
• Tiscornia, Gustavo
• Montserrat Pulido, Nuria
• Vassena, Rita
• Batlle Morera, Laura
• Rodriguez Piza, Ignasi
• Belmonte, Juan Carlos Izpisua

Titel

Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2009-06, Vol.106 (22), p.8918-8922

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.