Details

Autor(en) / Beteiligte

• Kari L. Stone
• Rachel K. Behan
• Green, Michael T.

Titel

X-ray Absorption Spectroscopy of Chloroperoxidase Compound I: Insight into the Reactive Intermediate of P450 Chemistry

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2005-11, Vol.102 (46), p.16563-16565

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2005

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• We report the structural characterization of a thiolate-ligated ferryl radical. Using x-ray absorption spectroscopy, we examined chloroperoxidase (CPO) compound I (CPO-I). Our results indicate that CPO-I is an authentic ferryl species with an Fe-O bond of 1.65 Å. Axial-ligand interactions result in a remarkably long 2.48-Å Fe-S bond. Analogous forms of cytochrome P450 and CPO have been shown to possess virtually identical coordination environments. Thus, it seems likely that our findings provide a good structural description of the elusive P450-I.