Details

Autor(en) / Beteiligte

• Sakchaisri, Krisada
• Asano, Satoshi
• Yu, Li-Rong
• Shulewitz, Mark J.
• Park, Chong J.
• Park, Jung-Eun
• Cho, Young-Wook
• Veenstra, Timothy D.
• Thorner, Jeremy
• Lee, Kyung S.
• Glazer, Alexander N.

Titel

Coupling Morphogenesis to Mitotic Entry

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2004-03, Vol.101 (12), p.4124-4129

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2004

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• In eukaryotes, cyclin B-bound cyclin-dependent protein kinase 1 promotes mitotic entry but is held in check, in part, by Wee1 protein kinase. Timely mitotic entry in budding yeast requires inactivation of Swe1 (Wee1 ortholog). Perturbations of the septin collar at the bud neck lead to Swe1 stabilization, delaying the G2/ M transition. Swe1 is recruited to the neck and hyperphosphorylated before ubiquitin-mediated degradation. Hsl1 kinase (Nim1 ortholog), a negative regulator of Wee1, is required for efficient Swe1 localization at the neck but seems not to phosphorylate Swe1. Here, we show that two other kinases targeted sequentially to the neck, Cla4/PAK and Cdc5/Polo, are responsible for stepwise phosphorylation and down-regulation of Swe1. This mechanism links assembly of a cellular structure to passage into mitosis.