Details

Autor(en) / Beteiligte

• Chanda, Sumit K.
• White, Suhaila
• Orth, Anthony P.
• Reisdorph, Richard
• Miraglia, Loren
• Thomas, Russell S.
• DeJesus, Paul
• Mason, Daniel E.
• Huang, Qihong
• Vega, Raquel
• Yu, De-Hua
• Nelson, Christian G.
• Smith, Brendan M.
• Terry, Robert
• Linford, Alicia S.
• Yu, Yang
• Chirn, Gung-wei
• Song, Chuanzheng
• Labow, Mark A.
• Cohen, Dalia
• King, Frederick J.
• Peters, Eric C.
• Schultz, Peter G.
• Vogt, Peter K.
• Hogenesch, John B.
• Caldwell, Jeremy S.

Titel

Genome-Scale Functional Profiling of the Mammalian AP-1 Signaling Pathway

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2003-10, Vol.100 (21), p.12153-12158

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2003

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Large-scale functional genomics approaches are fundamental to the characterization of mammalian transcriptomes annotated by genome sequencing projects. Although current high-throughput strategies systematically survey either transcriptional or biochemical networks, analogous genome-scale investigations that analyze gene function in mammalian cells have yet to be fully realized. Through transient overexpression analysis, we describe the parallel interrogation of ≈20,000 sequence annotated genes in cancer-related signaling pathways. For experimental validation of these genome data, we apply an integrative strategy to characterize previously unreported effectors of activator protein-1 (AP-1) mediated growth and mitogenic response pathways. These studies identify the ADP-ribosylation factor GTPase-activating protein Centaurin α1 and a Tudor domain-containing hypothetical protein as putative AP-1 regulatory oncogenes. These results provide insight into the composition of the AP-1 signaling machinery and validate this approach as a tractable platform for genome-wide functional analysis.