PloS one, 2023-07, Vol.18 (7), p.e0288362-e0288362
2023
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- Autor(en) / Beteiligte
- Titel
- Expression analysis of box C/D snoRNAs with SNPs between C57BL/6 and MSM/Ms strains in male mouse
- Ist Teil von
- PloS one, 2023-07, Vol.18 (7), p.e0288362-e0288362
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2023
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- MSM/Ms mouse derived from the Japanese wild mouse has unique characteristics compared to the widely used C57BL/6 mouse. To examine the usefulness of the MSM/Ms mouse for the comparative genomic analysis, expression of small RNAs were analyzed by the large-scale sequence analysis for two strains of mouse, C57BL/6 and MSM/Ms. As a trial, expression of box C/D snoRNAs, which are the most abundant small RNAs in the cell, were analyzed. By the comparison of the read number for each fragment, 11 snoRNAs with single nucleotide polymorphisms (SNPs) were detected. One of the snoRNAs, SNORD53, shows the expression only for MSM/Ms and this snoRNA has a mutation in the box sequence in C57BL/6. Thus, it was demonstrated that the proposed experimental system using SNPs can give new insight for the gene expression regulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0288362Titel-ID: cdi_plos_journals_2835426903
- Format
- –
- Schlagworte
- Analysis, Animals, Base Sequence, Biology and life sciences, Biotechnology industry, Brain, Comparative analysis, Gene expression, Gene mutations, Genes, Genetic aspects, Genomes, Genomic analysis, Genomics, Health aspects, Homosexuality, Male, Humans, Male, Mice, Mice, Inbred C57BL, MicroRNAs, Mutation, Nucleotides, Polymorphism, Single Nucleotide, Research and analysis methods, RNA, Small Nucleolar - genetics, Sequence analysis, Sexual and Gender Minorities, Single nucleotide polymorphisms, Single-nucleotide polymorphism, snoRNA