Details

Autor(en) / Beteiligte

• Lawrence, Anna-Lisa E
• Berger, Ryan P
• Hill, David R
• Huang, Sha
• Yadagiri, Veda K
• Bons, Brooke
• Fields, Courtney
• Sule, Gautam J
• Knight, Jason S
• Wobus, Christiane E
• Spence, Jason R
• Young, Vincent B
• O'Riordan, Mary X
• Abuaita, Basel H
• Baumler, Andreas J.

Titel

Human neutrophil IL1β directs intestinal epithelial cell extrusion during Salmonella infection

Ist Teil von

• PLoS pathogens, 2022-10, Vol.18 (10), p.e1010855-e1010855

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Infection of the human gut by Salmonella enterica Typhimurium (STM) results in a localized inflammatory disease that is not mimicked in murine infections. To determine mechanisms by which neutrophils, as early responders to bacterial challenge, direct inflammatory programming of human intestinal epithelium, we established a multi-component human intestinal organoid (HIO) model of STM infection. HIOs were micro-injected with STM and seeded with primary human polymorphonuclear leukocytes (PMN-HIOs). PMNs did not significantly alter luminal colonization of Salmonella, but their presence reduced intraepithelial bacterial burden. Adding PMNs to infected HIOs resulted in substantial accumulation of shed TUNEL+ epithelial cells that was driven by PMN Caspase-1 activity. Inhibition of Caspases-1, -3 or -4 abrogated epithelial cell death and extrusion in the infected PMN-HIOs but only Caspase-1 inhibition significantly increased bacterial burden in the PMN-HIO epithelium. Thus, PMNs promote cell death in human intestinal epithelial cells through multiple caspases as a protective response to infection. IL-1β was necessary and sufficient to induce cell shedding in the infected HIOs. These data support a critical innate immune function for human neutrophils in amplifying cell death and extrusion of human epithelial cells from the Salmonella-infected intestinal monolayer.