PloS one, 2022-09, Vol.17 (9), p.e0275168-e0275168
2022
Details
- Autor(en) / Beteiligte
- Titel
- SSNIP-seq: A simple and rapid method for isolation of single-sperm nucleic acid for high-throughput sequencing
- Ist Teil von
- PloS one, 2022-09, Vol.17 (9), p.e0275168-e0275168
- Ort / Verlag
- San Francisco: Public Library of Science
- Erscheinungsjahr
- 2022
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- We developed a simple and reliable method for the isolation of haploid nuclei from fresh and frozen testes. The described protocol uses readily available reagents in combination with flow cytometry to separate haploid and diploid nuclei. The protocol can be completed within 1 hour and the resulting individual haploid nuclei have intact morphology. The isolated nuclei are suitable for library preparation for high-throughput DNA and RNA sequencing using bulk or single nuclei. The protocol was optimised with mouse testes and we anticipate that it can be applied for the isolation of mature sperm from other mammals including humans.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0275168Titel-ID: cdi_plos_journals_2719396985
- Format
- –
- Schlagworte
- Analysis, Biology and Life Sciences, Chemical tests and reagents, Chloride, Cold, Deoxyribonucleic acid, Diploids, DNA, DNA sequencing, Engineering and Technology, Flow cytometry, Gene sequencing, Genetic aspects, Genomes, Genomics, Lab Protocol, Laboratories, Medicine and Health Sciences, Methods, Next-generation sequencing, Nuclei, Nuclei (cytology), Nucleic acids, Physical Sciences, Reagents, Research and Analysis Methods, RNA sequencing, Sperm, Spermatozoa, Spleen