PloS one, 2022-09, Vol.17 (9), p.e0274397-e0274397
2022
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- Autor(en) / Beteiligte
- Titel
- The regulon of Brucella abortus two-component system BvrR/BvrS reveals the coordination of metabolic pathways required for intracellular life
- Ist Teil von
- PloS one, 2022-09, Vol.17 (9), p.e0274397-e0274397
- Ort / Verlag
- San Francisco: Public Library of Science
- Erscheinungsjahr
- 2022
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Brucella abortus is a facultative intracellular pathogen causing a severe zoonotic disease worldwide. The two-component regulatory system (TCS) BvrR/BvrS of B. abortus is conserved in members of the Alphaproteobacteria class. It is related to the expression of genes required for host interaction and intracellular survival. Here we report that bvrR and bvrS are part of an operon composed of 16 genes encoding functions related to nitrogen metabolism, DNA repair and recombination, cell cycle arrest, and stress response. Synteny of this genomic region within close Alphaproteobacteria members suggests a conserved role in coordinating the expression of carbon and nitrogen metabolic pathways. In addition, we performed a ChIP-Seq analysis after exposure of bacteria to conditions that mimic the intracellular environment. Genes encoding enzymes at metabolic crossroads of the pentose phosphate shunt, gluconeogenesis, cell envelope homeostasis, nucleotide synthesis, cell division, and virulence are BvrR/BvrS direct targets. A 14 bp DNA BvrR binding motif was found and investigated in selected gene targets such as virB1, bvrR, pckA, omp25, and tamA. Understanding gene expression regulation is essential to elucidate how Brucella orchestrates a physiological response leading to a furtive pathogenic strategy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0274397Titel-ID: cdi_plos_journals_2716502289
- Format
- –
- Schlagworte
- Alphaproteobacteria, Alternative energy, Amino acids, Analysis, Bacteria, Binding sites, Biology and Life Sciences, Brucella abortus, Brucellosis, Carbon, Care and treatment, Cell cycle, Cell division, Cellular stress response, Deoxyribonucleic acid, Diagnosis, DNA, DNA repair, Gene expression, Gene regulation, Genes, Genomes, Gluconeogenesis, Health aspects, Homeostasis, Infections, Intracellular, Kinases, Maximum likelihood method, Medicine and Health Sciences, Metabolic pathways, Metabolism, Nitrogen, Nitrogen metabolism, Nucleotides, Outer membrane proteins, Pathways, Pentose, Pentose phosphate pathway, Phosphorylation, Proteins, Proteomics, Recombination, Research and Analysis Methods, Respiration, Signal transduction, Synteny, Virulence, Zoonoses