Details

Autor(en) / Beteiligte

• Funston, Wendy
• Ruchaud-Sparagano, Marie-Hélène
• Scott, Jonathan
• Powell, Jason
• Cooles, Faye A. H
• Shelmerdine, Lauren
• McDowell, Cliona
• O'Leary, Denis
• Booth, Karen L
• Clark, Stephen C
• Ledingham, Simon J
• Rostron, Anthony J
• Dark, John H
• Simpson, A. John
• Chen, Robert Jeenchen

Titel

A human model of bilateral pulmonary vein sampling to assess the effects of one-lung ventilation on neutrophil function

Ist Teil von

• PloS one, 2022-07, Vol.17 (7), p.e0271958-e0271958

Ort / Verlag

San Francisco: Public Library of Science

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Neutrophil activation drives lung complications after cardiopulmonary bypass (CPB). Evidence suggests the healthy, ventilated lung may beneficially re-condition pro-inflammatory neutrophils. However, evidence in humans is lacking, due to a paucity of good models. CPB with simultaneous central venous and bilateral pulmonary vein sampling provides an opportunity to model effects of one-lung ventilation. The study's primary objectives were to establish a model of intra-operative, bilateral pulmonary vein sampling and to determine whether neutrophil function differed after passing through inflated or deflated lungs. Seventeen patients having "on pump" coronary artery bypass grafting (CABG) with one-lung ventilation (in two cohorts with tidal volume 2ml kg.sup.-1 and FiO.sub.2 0.21, or tidal volume 4 ml kg.sup.-1 and FiO.sub.2 0.5 respectively) were recruited. Cohort 1 consisted of 9 patients (7 male, median age 62.0 years) and Cohort 2 consisted of 8 male patients (median age 65.5 years). Recruitment was via prospective screening of scheduled elective and non-elective CABG procedures with cardiopulmonary bypass. Each patient had five blood samples taken-central venous blood pre-operatively; central venous blood pre-CPB; central venous blood post-CPB; pulmonary venous blood draining the ventilated lung post-CPB; and pulmonary venous blood draining the deflated lung post-CPB. Neutrophil phagocytosis and priming status were quantified. Plasma cytokines were measured. Phagocytosis and priming were not significantly different in neutrophils returning from the ventilated lung as compared to the non-ventilated lung. Plasma IL-6, IL-8 and IL-10 were significantly elevated by CPB. The intra-operative, bilateral pulmonary vein sampling model provides unique opportunities to assess biological effects of interventions to one lung, with the other lung acting as an internal control. Single-lung ventilation during CPB had no significant effects on neutrophil function.