Details

Autor(en) / Beteiligte

• Saso, Wakana
• Yamasaki, Masako
• Nakakita, Shin-Ichi
• Fukushi, Shuetsu
• Tsuchimoto, Kana
• Watanabe, Noriyuki
• Sriwilaijaroen, Nongluk
• Kanie, Osamu
• Muramatsu, Masamichi
• Takahashi, Yoshimasa
• Matano, Tetsuro
• Takeda, Makoto
• Suzuki, Yasuo
• Watashi, Koichi
• Kawaoka, Yoshihiro

Titel

Significant role of host sialylated glycans in the infection and spread of severe acute respiratory syndrome coronavirus 2

Ist Teil von

• PLoS pathogens, 2022-06, Vol.18 (6), p.e1010590-e1010590

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been transmitted across all over the world, in contrast to the limited epidemic of genetically- and virologically-related SARS-CoV. However, the molecular basis explaining the difference in the virological characteristics among SARS-CoV-2 and SARS-CoV has been poorly defined. Here we identified that host sialoglycans play a significant role in the efficient spread of SARS-CoV-2 infection, while this was not the case with SARS-CoV. SARS-CoV-2 infection was significantly inhibited by α2-6-linked sialic acid-containing compounds, but not by α2-3 analog, in VeroE6/TMPRSS2 cells. The α2-6-linked compound bound to SARS-CoV-2 spike S1 subunit to competitively inhibit SARS-CoV-2 attachment to cells. Enzymatic removal of cell surface sialic acids impaired the interaction between SARS-CoV-2 spike and angiotensin-converting enzyme 2 (ACE2), and suppressed the efficient spread of SARS-CoV-2 infection over time, in contrast to its least effect on SARS-CoV spread. Our study provides a novel molecular basis of SARS-CoV-2 infection which illustrates the distinctive characteristics from SARS-CoV.