Details

Autor(en) / Beteiligte

• Akkad, Adam R
• Gu, Jian
• Duane, Brett
• Norquist, Alan
• Brenner, David J
• Ramakumar, Adarsh
• Zenhausern, Frederic
• Al-Ameri, Talib

Titel

Automatic reagent handling and assay processing of human biospecimens inside a transportation container for a medical disaster response against radiation

Ist Teil von

• PloS one, 2022-05, Vol.17 (5), p.e0268508-e0268508

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Biological materials can be shipped off-site for diagnostic, therapeutic and research purposes. They usually are kept in certain environments for their final application during transportation. However, active reagent handling during transportation from a collection site to a laboratory or biorepository has not been reported yet. In this paper, we show the application of a micro-controlled centrifugal microfluidic system inside a shipping container that can add reagent to an actively cultured human blood sample during transportation to ensure a rapid biodosimetry of cytokinesis-block micronucleus (CBMN) assay. The newly demonstrated concept could have a significant impact on rapid biodosimetry triage for medical countermeasure in a radiological disaster. It also opens a new capability in accelerated sample processing during transportation for biomedical and healthcare applications.