PloS one, 2022-05, Vol.17 (5), p.e0261699-e0261699
2022
Details
- Autor(en) / Beteiligte
- Titel
- Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system
- Ist Teil von
- PloS one, 2022-05, Vol.17 (5), p.e0261699-e0261699
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2022
- Link zum Volltext
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3-ITD protein was expressed in Bombyx mori L. pupae infected by gene-modified nucleopolyhedrovirus, and was purified as an active state. We performed an inhibition assay using 17 kinase inhibitors, and succeeded in screening two inhibitors for FLT3-ITD. The result has paved the way for screening FLT3-ITD inhibitors in a fast and easy manner, and also for structural studies.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0261699Titel-ID: cdi_plos_journals_2686217871
- Format
- –
- Schlagworte
- Antibodies, Antimitotic agents, Antineoplastic agents, Baculoviridae, Baculovirus, Baculoviruses, Biological products, Biology and Life Sciences, Bombyx mori, Chromatography, Drugs, Flt3 protein, Inhibitors, Kinases, Medicine and Health Sciences, Mutation, Peptides, Pharmaceutical research, Protein expression, Protein purification, Protein-tyrosine kinase, Proteins, Research and Analysis Methods, Screening, Separation, Silkworms, Tyrosine