Details

Autor(en) / Beteiligte

• Muruato, Antonio
• Vu, Michelle N
• Johnson, Bryan A
• Davis-Gardner, Meredith E
• Vanderheiden, Abigail
• Lokugamage, Kumari
• Schindewolf, Craig
• Crocquet-Valdes, Patricia A
• Langsjoen, Rose M
• Plante, Jessica A
• Plante, Kenneth S
• Weaver, Scott C
• Debbink, Kari
• Routh, Andrew L
• Walker, David
• Suthar, Mehul S
• Shi, Pei-Yong
• Xie, Xuping
• Menachery, Vineet D
• Cadwell, Ken

Titel

Mouse-adapted SARS-CoV-2 protects animals from lethal SARS-CoV challenge

Ist Teil von

• PLoS biology, 2021-11, Vol.19 (11), p.e3001284-e3001284

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2021

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• The emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a pandemic causing significant damage to public health and the economy. Efforts to understand the mechanisms of Coronavirus Disease 2019 (COVID-19) have been hampered by the lack of robust mouse models. To overcome this barrier, we used a reverse genetic system to generate a mouse-adapted strain of SARS-CoV-2. Incorporating key mutations found in SARS-CoV-2 variants, this model recapitulates critical elements of human infection including viral replication in the lung, immune cell infiltration, and significant in vivo disease. Importantly, mouse adaptation of SARS-CoV-2 does not impair replication in human airway cells and maintains antigenicity similar to human SARS-CoV-2 strains. Coupled with the incorporation of mutations found in variants of concern, CMA3p20 offers several advantages over other mouse-adapted SARS-CoV-2 strains. Using this model, we demonstrate that SARS-CoV-2-infected mice are protected from lethal challenge with the original Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), suggesting immunity from heterologous Coronavirus (CoV) strains. Together, the results highlight the use of this mouse model for further study of SARS-CoV-2 infection and disease.