PloS one, 2021-12, Vol.16 (12), p.e0261417-e0261417
2021
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Details
- Autor(en) / Beteiligte
- Titel
- Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect
- Ist Teil von
- PloS one, 2021-12, Vol.16 (12), p.e0261417-e0261417
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0261417Titel-ID: cdi_plos_journals_2610902426
- Format
- –
- Schlagworte
- Animals, Benzoates, Benzoates - pharmacology, Benzoic acid, Benzyl Alcohol - pharmacology, Biology and Life Sciences, Biopsy, Brain - drug effects, Clearing, Developmental biology, Histology, Pathological, Humans, Hydrogels, Imaging, Three-Dimensional - methods, Independent sample, Laboratories, Liver - drug effects, Male, Medical research, Medicine and Health Sciences, Methods, Microscopy, Neurology, Optical Imaging - methods, Physical Sciences, Prostate, Prostate - drug effects, Rats, Research and Analysis Methods, Shrinkage, Size effects, Solvents, Solvents - pharmacology, Spleen - drug effects, Swine, Testing, Tissue culture, Tissue Fixation - methods