Details

Autor(en) / Beteiligte

• Aubry, Maite
• Teiti, Iotefa
• Teissier, Anita
• Richard, Vaea
• Mariteragi-Helle, Teheipuaura
• Chung, Kiyojiken
• Deen, Farah
• Paoaafaite, Tuterarii
• Cao-Lormeau, Van-Mai
• Ito, Etsuro

Titel

Self-collection and pooling of samples as resources-saving strategies for RT-PCR-based SARS-CoV-2 surveillance, the example of travelers in French Polynesia

Ist Teil von

• PloS one, 2021-09, Vol.16 (9), p.e0256877-e0256877

Ort / Verlag

San Francisco: Public Library of Science

Erscheinungsjahr

2021

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• In French Polynesia, the first case of SARS-CoV-2 infection was detected on March 10.sup.th, 2020, in a resident returning from France. Between March 28.sup.th and July 14.sup.th, international air traffic was interrupted and local transmission of SARS-CoV-2 was brought under control, with only 62 cases recorded. The main challenge for reopening the air border without requiring travelers to quarantine on arrival was to limit the risk of re-introducing SARS-CoV-2. Specific measures were implemented, including the obligation for all travelers to have a negative RT-PCR test for SARS-CoV-2 carried out within 3 days before departure, and to perform another RT-PCR testing 4 days after arrival. Because of limitation in available medical staff, travelers were provided a kit allowing self-collection of oral and nasal swabs. In addition to increase our testing capacity, self-collected samples from up to 10 travelers were pooled before RNA extraction and RT-PCR testing. When a pool tested positive, RNA extraction and RT-PCR were performed on each individual sample. We report here the results of COVID-19 surveillance (COV-CHECK PORINETIA) conducted between July 15.sup.th, 2020, and February 15.sup.th, 2021, in travelers using self-collection and pooling approaches. We tested 5,982 pools comprising 59,490 individual samples, and detected 273 (0.46%) travelers positive for SARS-CoV-2. A mean difference of 1.17 Ct (CI 95% 0.93-1.41) was found between positive individual samples and pools (N = 50), probably related to the volume of samples used for RNA extraction (200 [mu]L versus 50 [mu]L, respectively). Retrospective testing of positive samples self-collected from October 20.sup.th, 2020, using variants-specific amplification kit and spike gene sequencing, found at least 6 residents infected by the Alpha variant. Self-collection and pooling approaches allowed large-scale screening for SARS-CoV-2 using less human, material and financial resources. Moreover, this strategy allowed detecting the introduction of SARS-CoV-2 variants of concern in French Polynesia.