PloS one, 2021-08, Vol.16 (8), p.e0255075-e0255075
2021
Details
- Autor(en) / Beteiligte
- Titel
- Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties
- Ist Teil von
- PloS one, 2021-08, Vol.16 (8), p.e0255075-e0255075
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Induced endothelial cells (iECs) generated from neonatal fibroblasts via transdifferentiation have been shown to have pro-angiogenic properties and are a potential therapy for peripheral arterial disease (PAD). It is unknown if iECs can be generated from fibroblasts collected from PAD patients and whether these cells are pro-angiogenic. In this study fibroblasts were collected from four PAD patients undergoing carotid endarterectomies. These cells, and neonatal fibroblasts, were transdifferentiated into iECs using modified mRNA. Endothelial phenotype and pro-angiogenic cytokine secretion were investigated. NOD-SCID mice underwent surgery to induce hindlimb ischaemia in a murine model of PAD. Mice received intramuscular injections with either control vehicle, or 1 × 106 neonatal-derived or 1 × 106 patient-derived iECs. Recovery in perfusion to the affected limb was measured using laser Doppler scanning. Perfusion recovery was enhanced in mice treated with neonatal-derived iECs and in two of the three patient-derived iEC lines investigated in vivo. Patient-derived iECs can be successfully generated from PAD patients and for specific patients display comparable pro-angiogenic properties to neonatal-derived iECs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0255075Titel-ID: cdi_plos_journals_2560042337
- Format
- –
- Schlagworte
- Acetylation - drug effects, Analysis, Angiogenesis, Animal models, Animals, Biopsy, Capillaries - drug effects, Cell Line, Cell Movement - drug effects, Cell Transdifferentiation - drug effects, Collagen - pharmacology, Culture Media, Conditioned - pharmacology, Cytokines, Cytokines - metabolism, Diagnosis, Diseases, Drug Combinations, Endothelial cells, Endothelial Cells - drug effects, Endothelial Cells - pathology, Endothelial Cells - transplantation, Endothelium, Fibroblasts, Fibroblasts - drug effects, Fibroblasts - pathology, Health aspects, Heart, Hindlimb - blood supply, Hindlimb - pathology, Humans, In vivo methods and tests, Infant, Newborn, Infants (Newborn), Intercellular Signaling Peptides and Proteins - pharmacology, Ischemia, Ischemia - pathology, Ischemia - therapy, Laminin - pharmacology, Lipoproteins, LDL - metabolism, Male, Medical research, Medicine, Experimental, Mice, Mice, Inbred NOD, Mice, SCID, mRNA, Neonates, Neovascularization, Physiologic - drug effects, Newborn babies, Patients, Perfusion, Peripheral Arterial Disease - pathology, Peripheral vascular diseases, Phenotypes, Plant Lectins - metabolism, Protein Binding - drug effects, Proteoglycans - pharmacology, Recovery, Type 2 diabetes, Vascular endothelial growth factor