Details

Autor(en) / Beteiligte

• Taylor, Roslyn A
• Xiao, Sixia
• Carias, Ann M
• McRaven, Michael D
• Thakkar, Divya N
• Araínga, Mariluz
• Allen, Edward J
• Rogers, Kenneth A
• Kumarapperuma, Sidath C
• Gong, Siqi
• Fought, Angela J
• Anderson, Meegan R
• Thomas, Yanique
• Schneider, Jeffrey R
• Goins, Beth
• Fox, Peter
• Villinger, Francois J
• Ruprecht, Ruth M
• Hope, Thomas J
• Douek, Daniel C.

Titel

PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure

Ist Teil von

• PLoS pathogens, 2021-06, Vol.17 (6), p.e1009632-e1009632

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Human immunodeficiency virus (HIV) vaccines have not been successful in clinical trials. Dimeric IgA (dIgA) in the form of secretory IgA is the most abundant antibody class in mucosal tissues, making dIgA a prime candidate for potential HIV vaccines. We coupled Positron Emission Tomography (PET) imaging and fluorescent microscopy of 64Cu-labeled, photoactivatable-GFP HIV (PA-GFP-BaL) and fluorescently labeled dIgA to determine how dIgA antibodies influence virus interaction with mucosal barriers and viral penetration in colorectal tissue. Our results show that HIV virions rapidly disseminate throughout the colon two hours after exposure. The presence of dIgA resulted in an increase in virions and penetration depth in the transverse colon. Moreover, virions were found in the mesenteric lymph nodes two hours after viral exposure, and the presence of dIgA led to an increase in virions in mesenteric lymph nodes. Taken together, these technologies enable in vivo and in situ visualization of antibody-virus interactions and detailed investigations of early events in HIV infection.