Details

Autor(en) / Beteiligte

• Bessaiah, Hicham
• Pokharel, Pravil
• Loucif, Hamza
• Kulbay, Merve
• Sasseville, Charles
• Habouria, Hajer
• Houle, Sébastien
• Bernier, Jacques
• Massé, Éric
• Van Grevenynghe, Julien
• Dozois, Charles M
• Oswald, Eric

Titel

The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli

Ist Teil von

• PLoS pathogens, 2021-05, Vol.17 (5), p.e1009617-e1009617

Ort / Verlag

San Francisco: Public Library of Science

Erscheinungsjahr

2021

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Urinary tract infections (UTIs) are a common bacterial infectious disease in humans, and strains of uropathogenic Escherichia coli (UPEC) are the most frequent cause of UTIs. During infection, UPEC must cope with a variety of stressful conditions in the urinary tract. Here, we demonstrate that the small RNA (sRNA) RyfA of UPEC strains is required for resistance to oxidative and osmotic stresses. Transcriptomic analysis of the ryfA mutant showed changes in expression of genes associated with general stress responses, metabolism, biofilm formation and genes coding for cell surface proteins. Inactivation of ryfA in UPEC strain CFT073 decreased urinary tract colonization in mice and the ryfA mutant also had reduced production of type 1 and P fimbriae (pili), adhesins which are known to be important for UTI. Furthermore, loss of ryfA also reduced UPEC survival in human macrophages. Thus, ryfA plays a key regulatory role in UPEC adaptation to stress, which contributes to UTI and survival in macrophages.