Details

Autor(en) / Beteiligte

• Palomba, Emanuela
• Tirelli, Valentina
• de Alteriis, Elisabetta
• Parascandola, Palma
• Landi, Carmine
• Mazzoleni, Stefano
• Sanchez, Massimo
• Galli, Alvaro

Titel

A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor

Ist Teil von

• PloS one, 2021-06, Vol.16 (6), p.e0248382

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.